[Download] "Modification of Biomolecules by Lipoxidation-Derived Aldehydes" by Yolanda Yuan # eBook PDF Kindle ePub Free
eBook details
- Title: Modification of Biomolecules by Lipoxidation-Derived Aldehydes
- Author : Yolanda Yuan
- Release Date : January 18, 2013
- Genre: Medical,Books,Professional & Technical,
- Pages : * pages
- Size : 23500 KB
Description
Accumulating evidence implicates a role of free radical-mediated oxidative stress in many degenerative disease states, including Alzheimer disease (AD), Parkinson disease (PD), chronic heart disease, liver disease, inflammation, and aging. Free radical-mediated oxidation of polyunsaturated fatty acids, present in membranes and lipoproteins, leads to generation of a diverse array of reactive aldehydes that can readily modify biomolecules such as protein and DNA. In this thesis work, we will demonstrate the modification chemistry of biomolecules including amino acids, proteins, and DNA nucleobases by lipoxidation (LPO)-derived aldehydes, mainly 4-hydroxy-2-nonenal (HNE), 4-oxo-2-nonenal (ONE), 2,4-decadienal (DDE), acrolein, and malondialdehyde (MDA). The goal of this work is mainly focused on identification through model studies of new modification adducts, which may be used as biomarkers to study lipid peroxidation and oxidative stress lesions. Elucidating chemistry of the formation of LPO-derived aldehydes-mediated adducts could also give hints to understand oxidative stress disease conditions at the molecular level. High performance liquid chromatography (HPLC), mass spectrometry (including LC/ESI-MS and MALDI-TOF MS), as well as independent synthesis are the common tools used in this thesis.